Serological investigation remains the primary approach to achieving satisfactory results in STDs identification in developing and under developed countries. However, the accuracy of the native antigen used in the current diagnostic kits has proven to be insufficient globally, so significant efforts have consistently been made to find alternative reagents as capture antigens/antibody. Consequently, multi-epitope peptides are promising diagnostic markers,with the potential for im-

Dr. Ragul Paramasivam

Doctoral research

on

Abstract

Development of recombinant multiepitope proteins based point of care test diagnosis for multiple sexual transmitted diseases in humans

-proving the accuracy of diagnostic kits. This research aims to performed a systematic review to assess the diagnostic accuracy of all reported recombinant antigens and multiple point-of-care rapid assay platforms for Chlamydia, Gonorrhea, Syphilis, Herpes, Acquired Immune Deficiency Syndrome and Hepatitis B detection according to standard methods and summarise test performance using meta-analysis to identify promising antigen for development of multiepitopic peptide for detection of Chlamydia, Gonorrhea, Syphilis, Herpes, Acquired Immune Deficiency Syndrome and Hepatitis B antigen and antibodies. Further to develop indirect IgG and IgM ELISA, Lateral flow rapid assay and IgM and IgG piezoelectric QCM diagnostic assay to detect STIs antibodies using multiepitopic peptides (MEP). The study was performed at Mekelle City, Ethiopia on over 200 susceptible serum samples. The performance of the multiepitopic antigen based IgM and IgG antibody detection assay was compared with gold standard assay and commercial assays for diagnostic accuracy. This study establishes the adaptivity and efficiency of synthetic linear multiepitope recombinant peptides as a single or combinational antigen alternate in sero-diagnostics Each of the six multiepitope peptide antigens were screened in three different diagnostic platform with 200 suspected patients’ samples. IgM and IgG detecting Indirect ELISA assay were able to clearly differentiate primary and secondary infections. The sensitivity and specificity of AIDS/HIV and Syphilis diagnosis were comparatively higher the Chlamydia and gonorrhea diagnostic accuracy, this may be due the smaller sample no or the last of a gold standard assay for a reliable comparison. Lateral flow assays produced similar results with Hepatitis B, HIV/AIDS and Syphilis were of about >99% sensitivity and specificity. Pooled ELISA Positive samples were screened with optimized QCM and observed that the massive frequency shift is observed in IgM QCM as the IgM Antibodies are bulkier antibodies with higher molecular weight than IgG. Also, a relatively gradual increase in the frequency shift can be observed as the increase in antibodies count as inferred from ELISA comparison.

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